Life in the lab

Laboratory skills

The laboratory skills programme is designed and delivered by real scientists to provide science students with an opportunity to gain hands on experience using real laboratory equipment.

The laboratory experience programme will also allow students to decide whether laboratory research lives up to their expectations (before deciding which undergraduate degree to apply for).

During this course students will learn transferable skills suitable for any laboratory work.

Course content

The course covers the following topics:

  • Laboratory Induction
  • Gene expression analysis by Quantitative Real Time PCR
  • Multicolour Flowcytometry and its applications in life sciences research
  • Analysis of apoptosis by Annexin – PI method
  • Basics of mass spectroscopy
  • Step by step guide of lineage tracing
  • Circular dichroism spectroscopy
  • Electrophoretic mobility shift to analyse DNA-Protein interaction
  • Fluorescence resonance energy transfer (FRET) to analyse protein-protein interaction

Who this course is for:

The course is for the following target:

  • Biology students
  • Chemistry students
  • Biophysics, biochemistry, molecular biology, cell biology students
  • All life sciences students
  • Any enthusiastic person who is interested in biological sciences and biological research
  • Pharmacy students

 

Learning outcomes

By the end of this course you should be able to:

  • Accurately, safely and appropriately use all the equipment regularly used in DNA manipulation, including balances, pipettes, electrophoresis and centrifuges
  • Prepare chemical solution and reagents to the precision appropriate to the task
  • Demonstrate knowledge of the biochemical basis underpinning the molecular biology techniques taught in the workshop
  • Independently clone any gene into a plasmid vector (from RNA extraction, reverse transcription, polymerase chain reaction, ligation, bacterial transformation, to DNA extraction, DNA mapping and primer design)
  • Transfect plasmids and silencing RNAs to over-express or knock down protein expression in a primary cell line, extract protein, assess and quantify expression using Western blotting
  • Carry out molecular biology experiments and interpret the results, designing a strategy to circumvent potential failed experiments

Certificates

You’ll receive a certificate at the end of your course.

Further information

If you have any questions about the course you can email at 

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